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Abstract Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are revolutionizing the management of metabolic and liver diseases, demonstrating effectiveness in controlling blood sugar levels and promoting liver repair

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To identify the sensor Cys residues of KEAP1 involved in direct cysteinylation by free Cys, we incubated the recombinant purified KEAP1 with free Cys and determined by mass spectrometry that Cys residues 77, 151, 226, 288, 319, 489, 583, and 613 were modified by cysteinylation in vitro (Fig

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For the MTT and neutral red assays, cells were plated at both 0.8 10 5 cells/ml 2.4 10 5 cells/ml to establish seeding density for subsequent experiments