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Water volume and post-dose fasting period had no apparent effect on t 1/2,semaglutide,day10 (geometric means ranged from 150 to 159 h)

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After purification using a cation exchange solid-phase extraction column, a substitution reaction occurs with L-alanine--naphthylamide under the catalysis of butyl chloroformate and Triethylamine , generating a compound with strong ultraviolet absorption