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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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Because of its involvement in multiple cellular signaling systems, GHK-Cu is frequently used in cell biology and biochemical research exploring how peptide-metal complexes influence structural and regulatory pathways

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