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Romero et al, 2017
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Scintigraphy remains the gold-standard technique for quantifying gastric emptying and allows measurement of both solid and liquid emptying, potentially concurrently, although frequently only solid emptying is assessed
The mixture was incubated in the dark at ambient temperature for 30 min
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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37 C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5-CGGTGCCCTGAATGAACTGC-3, reverse primer 5-CAGTCATAGCCGAATAGCCT-3) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)
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