Cytosolic ROS produced by NADPH oxidase 2 (NOX2) in innate immune cells damage pathogens via lipid and DNA oxidation 37
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Three-dimensional magnetization-prepared rapid gradient-echo imaging (3D MP RAGE)
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1) Harvest cells from a 50 mL culture by centrifugation at 4C (e.g., 5,000 rpm for 15 minutes)