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To this end, samples were reconstituted in 333 l of methanol/toluene solution (1:1 v/v ratio), and treated with 167 l of 0.5 M sodium methoxide and incubated at room temperature for 1 h

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Synthesis of A-Chain of Bovine Insulin and Partial Synthesis of Crystalline Bovine Insulin From Synthetic A-and Natural B-Chains