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We confirmed both in vitro and in vivo that specific combination of ligands of different transporters, especially alanine and glutathione, is more efficient than single ligands to facilitate the penetration of nanocarrierss across the BBB [24, 32, 33, 42]
Instead, consider using the single puncture threading technique or the multiple puncture technique
Couturier J et al
Itoh et al., 2004
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