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Side effects are minimal and typically include mild soreness at the injection site
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And as I said, were still learning more and more every day
Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
Any contaminating proteases or other enzymes that might have entered during handling become much less active in cold storage
Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
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