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Standard solutions of nitrates (NO 3 ), nitrites (NO 2 ) and hydrogen peroxide (H 2 O 2 ) were prepared in ranges of concentrations corresponding to their content in PTL up to the concentrations that, according to the literature 39,40,41 correspond to the minimum inhibitory concentrations (MIC)

Measurement of early and late apoptosis using annexin V and PI flow cytometry We used FITC Annexin V Apoptosis Detection Kit with PI (BioLegend, San Diego, CA) to quantitate the early and late apoptosis of RINm5F cells

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Therapeutic strategies targeting cellular ferroptosis hold promise for improving cellular function and promoting the healing of diabetic wounds

Nakamaru Y, Akahoshi F, Iijima H, Hisanaga N, Kume T