For purification, a 5 ml GSTrap FF (GE Healthcare) was equilibrated with at least five column volumes of reducing lysis buffer
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Freshly made solutions of equal volume 5,5-dithio-bis(2-nitrobenzoic acid) and glutathione reductase with concentrations of 0.33 g l 1 and 1.67 units per ml, respectively, were mixed with lysate to a final volume of 140 l
Class-100 sterile preparation environment for all IV formulations, with single-use medical-grade disposables used for every cannulation and infusion setupno reuse, no exceptions
It contains sulfer, a substance that is required for the production of the bodys most abundant natural antioxidant, glutathione