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At the end of the experiment, rats were weighed and sacrificed under ketamine (90 mg/kg)-xylazine (10 mg/kg) anaesthesia by exsanguination method

Researchers working with mouse models (2030g body weight) dosing at 12mg/kg should use 810mg/ml concentrations to keep injection volumes between 0.020.06ml, while protocols using larger rat models (250300g) can work comfortably with 5mg/ml concentrations that produce 0.10.2ml volumes

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