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While this can be worrying, it's usually normal during this stage of life
Effect of increasing the ionic strength and diluting the cell lysate in the presence and absence of BugBuster without sonication Induced cells were next used in triplicates and re-suspended independently in LSB-2 containing 75, 150 or 300 mM NaCl keeping all the other components (10 mM Tris, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF) intact, followed by incubation with BugBuster and processed without any sonication
Fatty-Acid Uptake and Handling Extracellular fatty acids can enter cells through a combination of diffusion and protein-facilitated transport
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10.1111/1523-1747.ep12469795 109 PuzaC.CardonesA
