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The protein was eluted from the column with the same buffer used for equilibration
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You forgot to put your peptide vial back in the fridge
Carter, W.O
Zhao J, Cheng YY, Fan W, Yang CB, Ye SF, Cui W et al (2015) Botanical drug puerarin coordinates with nerve growth factor in the regulation of neuronal survival and neuritogenesis via activating ERK 1/2 and PI 3K/Akt signaling pathways in the neurite extension process
10.1002/hep.32235 47 KojimaM.TakahashiH.KuwashiroT.TanakaK.MoriH.OzakiI.et al (2020)
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