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1 g of protein from each sample was loaded onto the enrichment column before switching in line with the analytical column at a flow rate of 300 L/min

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these measurements were repeated every 4 weeks until week 20, and every 8 weeks thereafter, until week 100 and week 104 (within 3 days either side of scheduled visit day)

It typically follows a consultation and blood test to confirm a deficiency

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Brighten: I think it's important for people to understand that, um, you know, as much as like, it is not a comfortable topic to be like, oh, you're overweight and that could be impacting your fertility