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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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Notes: Notes: The sample needs to be homogenized completely

34,49,50 These findings suggest that APAP-induced cell death may not strictly conform to conventional definitions of pyroptosis or other established modes of cell death, highlighting the complexity and distinct characteristics of liver injury induced by APAP

Bi vit di y s gi 12 vin ung trng da cha glutathione ang c nh gi cao v tin dng trn th trng, gip bn la chn sn phm ph hp vi nhu cu v tnh trng da ca mnh