Immunoblot analysis of total (T), pellet 1 (P1), supernatant 1 (S1), pellet 2 (P2) and supernatant 2 (S2) fractions using anti-SLC33A1 antibody at 1:5,000 dilution
Glutathione chelates these copper ions, converting them to Cu, which tyrosinase cannot bind
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Our data suggest that silencing of LS GLP-1R neurons diminishes the anorectic effect but preserves the nausea response induced by liraglutide (Figure 3 and Supplemental Figure 7, PR), implying the involvement of other brain regions in nausea
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