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Stir gently if needed

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The lysates were centrifuged (10.000 g , 30 min, 4 C) and the supernatants were loaded on equilibrated Ni-NTA agarose columns (Qiagen)

B12 helps convert fat into usable energy, while Methionine, Inositol, and Choline (MIC) assist the liver in breaking down and eliminating fat