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(4) Add 300 L of chloroform-methanol (V:V=2:1) into the centrifugal tube with the lower layer of solution, vortex for 30 s, and ultrasonic extraction for 10 min in an ice-water bath

In studies on rats with sarcoma 45, Thymalin at low doses suppressed tumor growth by 78% and induced regression in over half of the cases, linked to increased lymphoproliferative activity in the thymus (5)

doi: 10.1038/s41467-024-53496-8 27 GaubatzJ