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Circulating hormones were measured using enzyme-linked immunosorbent assay (ELISA
tribromoethanol)
Effect of angiotensin-converting enzyme inhibition and angiotensin II receptor blockers on cardiac angiotensin-converting enzyme 2
These studies indicate that GLP-1 modulates the release of several neurotransmitters including serotonin, dopamine, GABA, and glutamate, which may regulate depressive-like behaviors
Metformin : This medication, used to treat diabetes, has been shown to reduce the absorption of vitamin B12 in the body and lower serum vitamin B12 concentrations in the blood
Participants in the study also followed a diet and exercise program, including a reduced calorie intake and a healthy, balanced diet
