NNMT (Nicotinamide N-Methyltransferase) is a cytosolic enzyme that catalyses the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to nicotinamide producing 1-methylnicotinamide (1-MNA) and S-adenosyl-homocysteine (SAH) as reaction products
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The peptide samples were loaded on a nanoscale capillary reverse-phase C18 column using a high-performance LC (HPLC) system (Thermo Fisher Scientific UltiMate3000) and eluted by a gradient
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