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In addition, ROS levels were evaluated using an ROS assay kit (Jiancheng Bioengineering Institute, China) according to the manufacturers instructions
Recovery from ROS-induced toxicity was tested through MTT assay, in HeLa and SHSY-5Y cells pre-exposed to 10 M 3-AT for 15 min or 1 mM MPP + for 24 h respectively, followed by 250 M 15 min Sp treatment
This inexpensive test can indicate mold toxicity as a potential culprit before moving on to more expensive diagnostics
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In addition to TMLHE , Beaudet and colleagues have identified another X-linked gene, SLC6A14 , with a connection to carnitine: its protein is involved in carnitine transport across the blood-brain barrier
