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The tissue surrounding the injection point, which maintains a tightly regulated pH environment, responds to this disruption with the brief inflammatory signals that produce the stinging or burning sensation

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This timing can increase serum levels of beneficial compounds by as much as 50%

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However, its use in humans remains challenging because to allergic responses, discomfort at administration sites, and other serious toxic events including hemolysis, nonspecific cytotoxic effects, itching feeling, and hypersensitivity during therapeutic therapy