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doi:10.1073/pnas.1817885116
3.3 Marker-free genetic engineering of edible mushrooms for oral biopharmaceutical production The next step in advancing mushroom molecular farming is the development of marker-free, food-grade transformation systems tailored for oral biopharmaceutical delivery
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Similar, B-FAHF-2- treated peanut-polarized splenocytes had inhibited the production of IL-4 and IL-5 without causing cell cytotoxicity [30]
Sonication was performed using Diagenode BioruptorR pico (Leige, Belgium) for 7 cycles (30 s on/30 s off) to produce DNA fragments of 100300 bp in length
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