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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM

FIGURE 2 This oxidative stress environment can activate immune signalling pathways promoting neuroinflammation through mitochondrial dysfunction and impaired mitophagy, as observed in recent studies on ASD brains (Pinto Payares et al., 2024)

Proteome-wide survey unveils distinctively different target proteins in lead-sensitive CD4 + T cells and neutrophils To investigate the mechanism behind lead-induced toxicity in CD4 + T cells and neutrophils, we surveyed lead-induced protein expression changes and protein-small molecule interaction (PSMI) proteome-wide using LCMS based methods

Acknowledgements Project supported by the National Natural Science Foundation of China (30672748) and Shandong Administration of Traditional Chinese Medicine (2005-070)

But not all studies have found this