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8 GIP and GLP-1 receptors are coexpressed in overlapping but distinct neuronal and peripheral cell populations, 9,10 raising the possibility that simultaneous agonism of both receptors might produce additive or synergistic effects beyond what either pathway could achieve alone

PLoS One, 7(6):e40536

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SNAP/FLAG-hGLP-1R plasma membrane recycling INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto poly-L-lysine-coated black, clear bottom 96-well plates in full media, stimulated with vehicle or 100 nM exendin-4 for 1 hour at 37C to enable receptor internalisation, washed with PBS and incubated in 100 nM tetramethylrhodamine (TMR)-tagged exendin-4 (Ex-4-TMR) in full media for 1 hour or 3 hours at 37C to label any receptors that recycle back to the plasma membrane after agonist-mediated internalisation

This can lead to elevated blood glucose levels and potentially worsen underlying health problems

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