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It's important to note that these injections typically last just under a week, which is why regular administration is crucial for maintaining consistent results
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In Vitro Antibody Production and Enzyme-Linked Immunospot Assay (ELISPOT) To assess the effect of L-Cn on B cells, purified CD19 + B cells were cultured with or without L-Cn in the presence of CPG (2.5 g/mL, InvivoGen) and PWM (5 g/mL, Sigma-Aldrich) for 13 days
After recording OMRs, adult zebrafish were anesthetized in a 0.02% tricaine solution for several minutes until gill movements slowed, removed from the solution, blotted dry on a Kimwipe, and decapitated
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