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26 Insulin Secretion Assay According to the experimental design, five islets of uniform size from each group were pre-incubated in Krebs Ringer Bicarbonate-HEPES solution supplemented with 2.8 mM glucose for 30 min

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Inhibition of early apoptotic events by Akt/PKB is dependent on the first committed step of glycolysis and mitochondrial hexokinase