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MicroRNA141 inhibits the differentiation of bone marrowderived mesenchymal stem cells in steroidinduced osteonecrosis via E2F3

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Sections were deparaffinized in a xylene ethanol series and placed in Tris-EDTA (TE) buffer for antigen retrieval (10 mM Tris, 1 mM EDTA, 0.05% Tween, pH = 9.0) and then blocked in 5% BSA

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