The M1 resin was pack into a column and washed with 20 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 3 mM CaCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide
This preclinical data provides an excellent base for human safety and efficacy trials [25]
So the protocol sets the dose to land GHK-Cu in a sensible daily range (~1.67 mg), and the three recovery/anti-inflammatory peptides ride along at their lower 0.33 mg each
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Most of that loss occurs during the first 40 weeks, with the rate being highest between weeks 8 and 24 of therapeutic dosing