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A 500 um solution of Dihexa was prepared in acetonitrile and the collected liver microsomes were suspended in a solution of 0.5 mg/ml of 0.1 M Tris buffer at a pH 7.38
For a 1mL reconstitution, draw to the 1mL mark
Why the backward system
Refrigerate on arrival, and reconstitute only when ready for laboratory use
Gastrointestinal absorption of vitamin B 12depends on the presence of sufficient intrinsic factor and calcium ions
Alcoholism impairs B12 status, so counsel on alcohol use
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