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Researchers followed participants for nearly four years as part of the Semaglutide Cardiovascular Outcomes, or SOUL, Trial

[32] Choline supplementation had beneficial effects on fetal neurodevelopment and maternal placental function

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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

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Immunohistochemical staining The paraffin-embedded lung tissues from COPD patients or rats were cut into sections, which were baked at 60 C for 20 min