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This gives the powder a chance to hydrate before mixing
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-MSH related peptides: a new class of anti-inflammatory and immunomodulating drugs
Culture media from 24-hour incubated cells were collected, stored at -80 C, and analyzed for urea concentration using LC/MS/MS API3000, normalized to total cell protein
The very high molecular weight aggregates could not be resolved by treatment with DTT
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE 33 partner repository with the dataset identifier PXD068108
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