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GST isoforms (GSTM1, GSTP1, GSTT1) are expressed in the cytosol, mitochondria, and nucleus across multiple organs, including the liver, kidneys, lungs, brain, and placenta, exhibiting broad substrate specificity against electrophiles (molecules deficient in electron pairs) and ROS (2733)

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In vitro selection is used to isolate probes that exhibit sufficiently fast dissociation kinetics to achieve rapid, repetitive binding to their target, enabling discrimination of true target recognition events from non-specific background

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