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albicans antibody (1:600 in PBS with 0.1% BSA and 0.1% saponin, rabbit anti- Candida , BP1006, Acris Antibodies) overnight, washed with PBS, and incubated with a secondary goat anti-rabbit AlexaFluor488 (1:500 in PBS with 0.1% BSA and 0.1% saponin, Invitrogen A32731) for 1 h in the dark at RT

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ROS staining in Arabidopsis was performed as previously described 15

However, aging, stress, and disease decrease your glutathione levels, leading to a host of health issues