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RNA isolation and quantitative RT-PCR gene expression levels The total RNA from the kidney tissue was isolated using the TRIzol reagent (Invitrogen) [27], and the cDNA was synthesized following the manufacturers protocol, using 1 g RNA (Prime ScriptTM RT reagent Kit, Takara)
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Limitations of revisional surgery include invasiveness, cost, low scalability, heterogeneity and higher complication rates compared to primary MBS 33
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