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NNMT inhibition preserved nicotinamide substrate pool Reduced nicotinamide methylation conserved S-adenosyl methionine Elevated intracellular NAD+ increased sirtuin pathway activity Adipocyte metabolic shift altered lipid handling in cell models Core Areas of Scientific Investigation NNMT Enzyme Inhibition: NNMT activity is elevated in adipose tissue in obesity models

doi: 10.18632/oncotarget.13189 87 PinelliNRHurrenKM

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Analysis of adipogenic and osteogenic differentiation of human mesenchymal stem cells Human mesenchymal stem cells (MSC) (Promocell, Heidelberg, Germany) were maintained in culture under basal medium (Promocell) and then induced for adipogenesis and osteogenesis with corresponding differentiation media (Promocell) for 14 days with medium change every 23 days

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