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Inflammatory cytokines induce nitric oxide (NO) and ROS production for innate immune cells and microglial cells

Additionally, they reduce oxidative stress, which may slow aging and enhance overall cellular health

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To induce lung inflammation, the mice were anesthetized and were administered an intranasal instillation of a mixture containing LPS (5 mg/kg) and papain (10 units/mouse) dissolved in sterile PBS, with sample sizes of n = 2 and n = 3 for the two independent experiments