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The standard reference agonist is GLP-1, which is tested in each experiment at several concentrations to generate a concentration-response curve from which its EC 50 value and SE is calculated using GraphPad Prism
The single mutants were fertile with brood sizes comparable to wild-type, and their embryos hatched into young larvae, except for puf-11(q971) , which had a partially penetrant embryonic lethality (Figure 2B)
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This uneven redistribution produces sudden shifts in contour
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