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Then we found the predicted consequential pairing of target region (top) and miRNA (bottom) between IGF-1R and hsa-let-7c on the TargetScanHuman website (Fig
Proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and blotted with anti-hCystatin C (MAB1196 [R&D, Minneapolis, MN] used at 500 ng/ml), and developed by enhanced chemiluminescence (ECL, Thermo Fisher Scientific)
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V oCysb , V oGlyb , and V oGlutb are inputs to the model
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