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Once reaching near-confluence, culture medium was aspirated and cells were washed once with PBS, followed by a 20 min incubation with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei
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Kochevar is very careful about adding too many calories with condiments like mayonnaise and salad dressing, so he uses them minimally
*nutrient reference values Fibersol is a registered trademark of Matsutani Chemical Industry Co., Ltd
You should store it at room temperature between 59F and 85F
Chronic obstructive pulmonary disease: molecular and cellular mechanisms
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