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This comprehensive process, utilizing reagents and instruments from various suppliers including Shenggong (Shanghai) for xylene and paraffin, Dingguo (Beijing) for 4% paraformaldehyde, and both Shenggong and Dexinkang for alcohols, ensures a detailed morphological assessment of the brain tissues response to treatment

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Only acceptable for very short-term holding before reconstitution

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Following a 10-min incubation at RT, absorbance was measured using a plate reader (Spectramax Plus 384) set to a wavelength of 530 nm