2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)
Briefly, NSCs were incubated at 37 for 15 min in PBS with 10 M DCFH-DA solution, followed by PBS washing three times
Biochemistry of human skin--our brain on the outside.Chemical Society Reviews
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