Briefly, 0.8 g plasmid DNA template was incubated with 40 l TnT T7 Quick Master Mix, 20 M Methionine and 2 l Transcend Biotin-Lysyl-tRNA (L1170, Promega) per 50 l reaction for 60 min at 30 C
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Additionally, it can be hypothesized that E1B-55K expression may indirectly cause the relocalization of IKK and NEMO by deregulating specific cellular proteins after its expression
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