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See the Wolverine Stack guide for combined research-planning context

Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

Topical serums Copper peptide serums are the most common format and the most likely to cause purging

Once you add bacteriostatic water, the clock starts ticking on a 28-30 day shelf life

Do not refreeze after reconstitution , crystallization during freezing/thawing can damage the peptide structure

Biochem J 196:363367 Shaheen SO, Rutterford CM, Lewis SJ, Ring SM, Holloway JW, Golding J, Henderson AJ (2015) Maternal selenium status in pregnancy, offspring glutathione peroxidase 4 genotype, and childhood asthma