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These intestinal sections were flushed with ice-cold phosphate-buffered saline (PBS) to remove fecal matter and placed in 1- to 2-cm pieces into 15 mL sterile tubes with 5 mL dissociation buffer (PBS, 10 mM EDTA) for incubation at 37C in a shaker at 250 rpm for 20 minutes

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Your provider will individualize this based on your tolerance, side effect history, and how much weight you still want to lose

This trial was important because a significant clinical benefit was seen in a lower-risk patient population irrespective of baseline cardiovascular risk