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In fact, LC protects against ROS-related harm to DNA and cell membranes
From a 5 mg vial reconstituted with 3 mL (1.67 mg/mL), 100 mcg is 0.06 mL = 6 units on a U-100 syringe
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Membranes were subsequently blocked with 5% non-fat milk in TBST buffer for 2 h at room temperature and incubated overnight at 4 C with the appropriate primary antibodies as follows: anti-Bcl-2 antibody, anti-BAX antibody, anti-Cleaved Caspase 3 (Cl-Caspase 3) antibody, and anti-GAPDH antibody
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Upon reaction with ROS or electrophiles, GSH becomes oxidized to GSSG, which can be reduced to GSH by the GSSG reductase (GR)
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