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After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)

Less than that and you dont get the full benefit of the product, and over 20 percent, you dont get any additional benefits and are more likely to experience irritation

Biogenetically, the expression of the master regulator PGC-1 and its downstream pathways retains functional potential, sustaining the capacity for mitochondrial biogenesis (38)

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Wawrzyski et al., 2006)