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Four groups

On the other hand, certain Lactobacillus species prevented barrier disruption through the upregulation of tight junction proteins

Follow your institution's SOPs and the manufacturer's package insert

At varying time points (0, 2, 5, 15, 30 min), purified Pt GSTF8 (8 M) and GSH (20 M) were added to an aliquot of the equilibration reaction and the resulting mixtures were incubated at 30 C for 30 min at 1200 rpm using a 3 mm shaking diameter before extraction with 2 vol of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid

Nieto-Bona et al., 1997)

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