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The pelleted crypts were resuspended in 25 l/well growth factor reduced Matrigel (Corning, New York, USA)
If you see any cloudiness, discoloration, or particulates in the water, do not use it
Concentrated (10 mg/mL) minimises injection volumes but requires precise measurements
PMCID: PMC4508379
For most patients, minimal preparation is required before receiving a Vitamin B12 shot
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