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Serum cytokine assays The serum IL-1, IL-6 and TNF- levels were detected by ELISA (Abebio, Wuhan, China) following the manufacturers instructions

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hence, nitro-oxidative stress is also thought to be one of the responsible factors of nervous degeneration that characterize PDN (Mirshekar et al., 2010

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The initiation of this early immune response depends on the recognition of certain viral structures known as pathogen-associated molecular patterns (PAMPs)

This discovery revealed GHK as a critical signaling molecule with high affinity for copper(II) ions, forming a stable complex essential for numerous biological functions